dCas9-KRAB mRNA (m1ψ substitution)
dCas9-KRAB mRNA encodes the dCas9-KRAB fusion protein, a tool for gene silencing and transcriptional repression.
The dCas9 (dead Cas9) component targets DNA precisely without cutting, while the KRAB (Krüppel-associated box) domain recruits complexes to inhibit gene expression. Delivered as mRNA, dCas9-KRAB ensures rapid, high transient expression without nuclear uptake, making it ideal for research in gene function, genetic diseases, and therapeutic development.
Croyez’s dCas9-KRAB mRNA is produced through in vitro transcription, featuring a Cap1-modified 5' end, a poly(A) tail at the 3' end, and modified nucleotides to reduce immune response. These enhancements ensure stability and performance, allowing the mRNA to function like natural mature mRNAs in cells.
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mRNA Length:
4982 nt
Base Composition:
N1-Me-pUTP (N1-mψ)
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Gene function research, gene regulation studies, therapeutic development, epigenetic research.
Shipping Conditions:
Dry ice
4982 nt
Base Composition:
N1-Me-pUTP (N1-mψ)
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Gene function research, gene regulation studies, therapeutic development, epigenetic research.
Shipping Conditions:
Dry ice
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Figure 1.
dCas9-KRAB mRNA (m1 ψ substitution) was analyzed on a 2% E-Gel.
Lane 1: Marker,RNA ladder (cat no: CR00004)
Lane 2: dCas9-KRAB mRNA (m1 ψ substitution)
.jpg)
Figure 2.
100 ng RNA + 4 mL RNA loading dye+ 1 mL novel juice, 5 min , 65℃ loading 6 mL to 1% TAE agarose gel, 120 V 30 min
Lane 1: Marker, RNA ladder (cat no: CR00004)
Lane 2: dCas9-KRAB mRNA (m1 ψ substitution)
